The level of native cdna of cytokine is determined by comparison with the known amount of competitor in agarose electrophoresis . the results from the expressions of cytokine from normal and immunized mice may be used to analyze the immune response profile or guide to design a better vaccine 结果表明能够初步定量免疫前后小鼠体内的il - 2 、 il - 4 、 il - 10 、 il - 12和ifn -的表达量，为深入研究基因疫苗免疫前后小鼠体内各种细胞因子的动态水平和分析细胞免疫效果奠定了基础。
